Wolbachia, an endosymbiont has been reported to act on the immune responses of its host and hinder other pathogenic microbes. Keeping this in view, it was necessary to screen and characterize Wolbachia. Hence it was essential to know, can Wolbachia infection be established in Human cell lines to study the effect of Wolbachia in uninfected Human cell host or to investigate Wolbachia – host cell interactions. Thus, in this study, we isolated Wolbachia and inoculated into the cultures of MRA 975 Human hepatocyte cell lines to examine the effect of Wolbachia in Human hepatocyte cells. Wolbachia infection of cells were assessed by Polymerase Chain Reaction (PCR) Assay, which indicated that MRA 975 Human hepatocyte cells was not infected with Wolbachia. So, our study suggests that Wolbachia cannot be infected, cultivated and established in MRA 975 Human hepatocyte cells to study the effect of Wolbachia or to investigate Wolbachia – host cell interactions.
Kishore. V. P et al.2016, Analysis of Human Hepatocyte Cell Lines For In Vitro Culture Andestablishment of wolbachia. Int J Recent Sci Res. 7(11), pp. 14177-14180.