The pharmacology of the Siddha system has three chief divisions the plant pharmacology, mineral pharmacology and animal pharmacology. The plant pharmacology has more than 530 herbals explained. The analytical techniques were planned to elucidate bioactive efficacy of Alpiniaofficinarum the crude, aqueous and ethanolic fractions isolated from Alpiniaofficinarum was evaluated against pathogenic bacteria. The crude samples were further screened using thin layer chromatographic method and confirmed by spectrometric analysis. The atomic absorption spectrometric analysis showed that Alpiniaofficinarum rhizomes contain essential elements like Cu, Zn & Mg and the heavy metals were below permissible limits. The ethanolic extraction of Alpiniaofficinarum rhizomes has been standardized. Thin layer chromatographic analysis shows the presence of 4 diffenrent active principles in ethanolic extract. The GC MS analysis confirms the presence of active components. The absorbance maxima (l max) studies of ethanolic extract characteristically was in the terminal UV range substantiates the presence of flavonoids and heptanoids in the extract. The observed antibacterial activity against E coli, Salmonella, Staphylococcus, Klebsiella and Proteus enumerates its bio efficiency and validates its potential use in Siddha system of medicine.
