Objective: Targeting antioxidants for treatment of oxidative stress mediated disease is a potential new therapeutic strategy. Antioxidants from phytochemicals ameliorating oxidative stress-mediated diseases possess their future promise for treatment. Present study was to assess antioxidant and free radical scavenging activities ofDiosgenin by using various in-vitro scavenging assays. Methods: 1, 1-diphenyl-2-picrylhydrazyl (DPPH), 2, 2-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) ABTS, hydroxyl, superoxide, hydrogen peroxide, nitric oxide radicals and reducing power scavenging assays. Results: The results of the study were showed that Diosgenin exhibited substantial inhibition of free radical which was calculated as IC50 value, compared with standard ascorbic acid. IC50 values of Diosgenin and ascorbic acid were found to be 46.14µg/mL and 39.27µg/mL in DPPH radical, 35.17µg/mL and 39.14µg/mL in ABTS●+, 34.21µg/mL and 38,24µg/mL in hydroxyl radical, 29.17µg/mL and 34.19µg/mL in superoxide anion radical, 32.12µg/mL and 36.17µg/mL in hydrogen peroxide radical, 35.19µg/mL and 39.14µg/mL in nitric oxide radical, and the reducing power of Diosgenin is 41.15µg/mL we compared to ascorbic acid 43.51 µg/mL.Conclusion: We conclude that above results showed Diosgenin has more potent antioxidant and free radical scavenging properties.
