The earthworm Eudrilus eugeniae is segmented worm which have the remarkable ability of regeneration. Organogenesis and animal adaptation upon loss of body parts are crucial events in regeneration of all traits and both are uncovered myths in biology. Earthworms so called ecological engineers are the sources of food, medicine and so on. In the present study the earthworm regenerated from the posterior end within a week. The regenerating tissue when tested for total antioxidant capacity and free radical scavenging activity, showed a remarkable result with high antioxidant and free radical scavenging activity. Free radical scavenging activity of Eudrilus eugeniae tissue using DPPH method was found to increase with increase in concentration of the sample. Thus from the current study it can be concluded that Eudrilus eugeniae can be utilized as good source of antioxidant potential and free radical scavenging activity.