An agglomerate multivariate study of the effect of crude oil exploration and exploitation on the surface water quality of Anakpa village stream 1 was conducted. This was done because Crude oil exploration and exploitation activities often introduce chemical substances which may contaminate the environment with trace metals. Therefore, to evaluate the extent of impact of such activities in the village stream of Anakpa principal component analysis method was utilized. The PCA was applied so as to investigate and identify sources of trace metals Fe, Zn, Pb, Cr, Cd, Vi and Ni in the surface water. The Study applied HACH Standard approved method procedure to determine the trace and nutrient contents of the domestic water. The study was conducted in village stream1 of Anakpa Uruan close to the area where crude oil exploration and exploitation is undertaken. Samples were collected randomly from upstream, downstream of the village stream 1. There were positive and insignificant relations between trace metals and nutrient content of the surface water samples as revealed in the multivariate analysis of the test sample results. Iron and PH correlated positively at p<0.05 with r value of 0. 689. Cd also exhibited positive correlation with phosphate at p<0.05 with r value of 0. 672.Pb also exhibited positive correlation with BOD at p<0.05 with r value of 0. 745. Other metals such as Nickel, Vanadium and chromium exhibited negative correlation with other nutrient content of the surface water tested. The result of agglomerate hierarchical analysis shows that the crude oil exploitation had serious effect on the enhancement of the trace metals load in the surface water of village stream1 in Anakpa, Uruan Local Government Area of Akwa Ibom State, Nigeria
