The present study has earnestly made an attempt to explore the relationship between selected anthropometric and physical fitness variables with the performance of athletes of 800 meter race and also attempted to find out the prediction equations of playing abilities of athletes. The subjects for the present study consisted of 30 college level girls athletes 18-25 years of age. Random sampling technique was adopted to collect the required data of the subjects at the time of inter college athletic meets, coaching camps and during athletics competitions. Thirty seven anthropometric variables and six physical fitness components were selected for the study. To collect the data for physical fitness of college girls, AAHPER Youth Fitness Test (1976) was used. To achieve the objectives of the present study, the inter-correlation was calculated by product moment method and multiple correlation and regression prediction equation by Worry Do Little method. The relationship between selected anthropometric variables performance was established by computing pearson’s product moment coefficient of correlation equation of performance. From the results it has been found that Weight is negatively correlated with performance whereas no relationship has been established between linear measurements and performance. Among girth measurements Shoulder, hip and thigh girths have recorded significantly negative correlation with performance. Triceps skinfold has been found positively and significantly correlated to performance among skinfold measurements. Significantly negative relationship has been found between percentage fat and lean body mass with performance among body composition variables. Flexibility has been significantly correlated to performance among the components of physical fitness. Multiple correlation of weight, hip girth, triceps skinfold, flexibility and percentage fat taken together with performance has been found significant. It can be concluded that anthropometric and physical fitness attributes effect the running performance of 800 meter race.