Introduction: Individuals who paint often complain to doctors about respiratory problems. Car and furniture painters are exposed to isocynate, especially HDI and HDI-BT. Isocyanate are powerful irritants to mucous membranes of eyes and gastrointestinal and respiratory tracts. It can sensitize workers making them subject to severe asthma attacks and other small airway diseases if they are exposed again. Excessive short term isocyanate exposure during work may produce greater risk. One of the most largest group who are heavily exposed to isocynate are spray painters. Peak expiratory flow rate is a persons maximum speed of expiration which can be measured with a peak flow meter. It measures the airflow through the bronchi and thus the degree of obstruction in the airway. Method: It is a type of observational study in which convenient sampling method was used. 200 subjects were taken according to the inclusion criteria. Subjects were assessed for height and peak expiratory flow rate of the subjects were taken in standing position. Three readings were taken and then best of three readings was noted down. These readings were compared with normal standard values. Results: After 4 months of study, data was analyzed using the normal standard value which showed statistical significant difference in peak expiratory flow rate of spray painters.(P < .05) Conclusion: There is significant differerence in the peak expiratory flow rate of spray painters and standard normal values.