Introduction: Indiscriminate use of antibiotics has led to a state where multi drug resistant bacteria have become increasingly prevalent. The knowledge of antibiotic resistance patterns is thereby necessary to stop the emergence of multi drug resistant bacteria. Material and methods: 1401 gram negative isolates from various clinical samples such as pus, urine, sputum, blood etc., were tested for the bacteriological profile and antibiogram in the Department of Microbiology, RIMS from April 2016 to March 2017. Results: Gram Negative Bacilli was isolated from 1401 samples, 762 were from male and 639 were from female patients. Escherichia coli was the most common gram negative isolate (414), followed by Klebsiella spp(123). Fosfomycin (1.61%) were the most sensitive drug and AMC (68.5%) were most resistant in the organisms isolated from urine. Imipenem (13.34%) followed by gentamicin were the most sensitive drugs for the gram negative bacilli isolated from samples other than urine. Conclusion: Empirical and appropriate use of antibiotics is very crucial in preventing emergence of multidrug resistant bacteria and the findings of our study will help clinicians for right and appropriate antibiotic choice in treating infections caused by gram negative organisms.
