The study was carried out to evaluate the biological activity (Anti-inflammatory, antioxidant and anti-cancer) of crude extract from S.tuirus isolated from Sankarabarani river estuary, Ariyankuppam–Puducherry. The active fraction of S.tuirus was obtained by solvent extraction method and was further confirmed by GC-MS. Free radical scavenging assay and the antiinflammatory activity of crude extracts of S.tuirus were also assessed. In vitro anticancer activity was examined by MTT assay. GC-MS analysis revealed that the fractions contained totally 25 bioactive compounds which included thiophene, 2-propyl and 2-acetoxy-5-nitrobenzaldehyde represented by a unique spectra. In vitro free radical scavenging activities of DPPH, FRAP (91%), NO (87%) and H2O2 (87%) by the crude extract were of S. tuirus were confirmed. In antiinflammatory activity assay, 99.08% of inhibition action was observed and the anticancer activity with an IC50 value of 222.30 µg/ml denoted that the active fraction contained a spectrum of biological activity.Isolation and characterization of the potential S.tuirus from the study place was endowed with enormous biological applications on an remarkable scale
