The present investigation was conducted to develop a protocol for rapid callus induction. Tephrosia hookeriana Wight & Arn was collected from Ayyalur, Dindigul District. The explants were cultured on Murashige and Skoog’s (MS) medium. The callus culture from leaf, node and internode explants in MS medium supplemented with different concentration and combination of 2, 4-D, NAA, IAA. The highest Percentage (92.35%) of callus from leaf explants was obtained by supplementation of (BAP-0.25mg/L-1 + 2, 4-D-2.00mg/L-1) and the lowest percentage of callus (8.00%) was obtained with the addition of (IAA-1.00mg/L-1) in MS medium. The callus was responded in 30 days from the leaf, node and internode explants. Different colors such as brown, green, greenish white, yellowish green, greenish yellow and brownish white callus were formed. This callus induction protocol was standardized from different explants for successful production of plant lets. There are no earlier reports on in vitro characterization of T.hookeriana.
