We are living in the viral world. Smallest the organism and greatest the disease. Virus is non living when present outside but living when present when present inside the cell. There are many types of viruses that cause a wide variety of viral diseases. The diagnosis of viral infection remains a major challenge in developing therapeutics. The clinical diagnosis provides state of the art for developing diagnostics for treatment (1). The main hurdle in developing diagnostics for the virus is their genome nature and high-level genome plasticity (2). To develop diagnostic for viral infection, there have been tremendous efforts have been over a period of several decades. The microscopy remains a major tool for morphological identification and validation of viruses. The advancement of cutting edge microscopy including electron microscopy, confocal microscopy, and scanning probe microscopy provide ease in identification and characterization of these intracellular pathogens (3). There have been continuous developments in Transmission electron microscope (TEM), scanning electron microscope (SEM), Atomic force microscope (AFM), scanning near-field optical microscope (SNOM), X-ray microscope, and ultrasonic microscope to enhance viral diagnosis (4).
