Background and Objectives: To evaluate and compare the salivary calcium, phosphate, amylase, total protein concentrations, flow rate, pH and buffering capacity before and during orthodontic treatment. Methods: Salivary samples were collected from 40 individuals in the age group of 15-25 years having malocclusion and comparison of salivary calcium, phosphate, amylase, total protein concentrations, flow rate, pH and buffering capacity was done 45 days after beginning the orthodontic treatment. Results: Salivary alpha-amylase, calcium, and phosphate concentrations were higher in patients undergoing orthodontic treatment as compared to those patients before starting treatment. Interpretation and Conclusion: The salivary parameters such as pH, buffering capacity, flow rate were significantly reduced in patients undergoing orthodontic treatment whereas parameters such as total protein concentration, salivary calcium, phosphate and amylase were increased.
