The large amount of genomic information present in the global databases may be analyzed from a wide variety of perspectives to obtain novel information regarding the functional relation between different organisms as well as their comparative physiology, and lifestyle. In this study, an in-depth comparative codon utilization analysis has been employed to study the genome design of an interesting bacterial species Myxococcus, utilizing the whole genome of four myxococci species. Myxococcus represents the myxobacteria characterized by gliding motility, fruit-body formation and the production of lytic principles capable of attacking different bacterial and fungal cells on which the formation of fruiting bodies readily takes place. Among the four species analyzed in this study, M. fulvus was found to display the most codon biased genome with ninety percent of its protein coding genes having Nc values below 40. Correlation between the different codon usage parameters showed strong negative correlation between Nc and GC3; the GRAVY score, which is a measure of hydropathicity of a protein was also found to be influenced by the genic GC content. An interesting observation of this comparative intra-specific codon usage utilization study was the relatively weak positive correlation between GC3 and GC content in the protein coding genes of the four Myxococcus species
