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In recent years, the incidence of fungal infections has been rising all over the world. The ability of Candida albicans to switch from yeast to hyphal growth is essential for its virulence. The aim of this comparative study was to biotype and characterizes phospholipase, proteinase, phosphatase and haemolytic activities of yeast and hyphal form of Candida albicans. The hyphal form of Candida albicans secrets high quantity of hydrolytic enzymes than yeast form which helps in its virulence. These results suggest that pathogenic fungi produce larger amount of inducible hydrolytic enzymes than non pathogenic fungi. In this investigation, we were used plate methods to determine phospholipase, proteinase and haemolytic activities and spectrophotometric method for acid phosphatase activity.