Comparitive Study On Efficacy Of Alkaline And Acid Extraction For Pha Recovery From Bacillus Subtilis Ncdc0671

Research Article
Mridul Umesh and Mamatha C
DOI: 
http://dx.doi.org/10.24327/ijrsr.2018.0911.2868
Subject: 
science
KeyWords: 
Polyhydroxyalkanoates, biocompatibility, biodegradability, recovery methods.
Abstract: 

The increasing global concern regarding the environmental hazards caused by the use and careless discharge of synthetic plastics has led to the increasing focus on bioplastics that are nontoxic and ecofriendly. Polyhydroxyalkanoates are a class of truly biodegradable polymers with material properties closely resembling their synthetic counterparts. Their biodegradability and biocompatibility had made them widely useful for application in medicine and industry. In spite of their potential application the major factor that hinders its commercialization is the production cost contributed significantly by extraction process for PHA recovery. This research work focuses on screening rapid and simple acid and alkali based method for PHA production. Further the effect of incubation temperature, concentration of acid/alkali and time period of treatment on PHA recovery was also assessed. The results obtained from the present study revealed that maximum PHA recovery of 76.53 ± 0.27% was obtained when the bacterial biomass was treated with 0.5 M NaOCl concentration.