In the present study, the karyotype of two teak defoliator sps. of, Eutectona, Hyblaea was studied from Jammu region, India. Chromosomal studied were made from testis by using 2% Giemsa air drying method. The chromosomes at metaphaseI stage appeared almost of similar size. The haploid chromosome numbers observed in E machaeralis and H purea were 18 and 31, respectively. The largest chromosome was 1.3µm and 0.9 µm in length while the shortest chromosome was 0.4µm &0.3µm respectively. Centromeric fusion of chromosome in these species must played active role leading to decrease in diploid chromosome number.