Degradation Of Low Density Polythene By Achromobacter Denitrificans Strain S1, A Novel Marine Isolate

Research Article
Ambika Devi K, Lakshmi B.K.M and Hemalatha.K.P.J
DOI: 
xxx-xxxxx-xxxx
Subject: 
science
KeyWords: 
Achromobacter denitrificans strain S1; LDPE; Degradation; FAME; GCMS; ESI-MS; NMR; and XRD.
Abstract: 

The present article reveals the biodegradation of low density polythene by bacterial strain isolated from marine water near Visakhapatnam. The degradation ability of the bacterial strain was evaluated by performing polythene zone of clearance method. The bacterial strain was identified and confirmed, by 16S rRNA gene sequencing and phylogenetic analysis, as Achromobacter denitrificans strain S1 (MTCC 12203). Lipid profiling was done by FAME analysis using GCMS and ESI-MS. Then polythene degradation ability of the strain was evaluated by measuring the dry weight loss in low density polythene (LDPE) films (20 and 40micron thickness). The LDPE degradation was further confirmed by tensile testing, NMR, XRD, thermo gravimetric, carbon analysis, and GCMS analysis.