The present Article portrays simple, sensitive, accurate, precise and cost effective First order derivative Spectrophotometric method and RP-HPLC method for the simultaneous estimation of Aripiprazole and Clozapine in Synthetic Mixture. In The first order derivative method absorption at 227.79 nm (zero crossing point for Clozapine) was used for Aripiprazole and 310.425 nm (zero crossing point for Aripiprazole) was used for Clozapine. The linearity was taken in the concentration range of 1- 5 µg/ml for Aripiprazole and 10-50 µg/ml for Clozapine with correlation coefficient (R2) 0.996 and 0.999, respectively. For The RP-HPLC method linearity was taken in the concentration range of 1- 5 µg/ml for Aripiprazole and 10-50 µg/ml for Clozapine with correlation coefficient (R2) 0.998 and 0.998, respectively. Proposed technique has been validated as per ICH guideline and successfully applied to the simultaneous estimation of Aripiprazole and Clozapine in their Synthetic Mixture. The results of analysis have been validated statistically and by recovery studies.
