Diazinon Induced Neurobehavioral Alterations and Histological Changes in Female Wistar Rat : Protective Effect of Pregnancy

Research Article
Hakima Tayaa*, Imed Eddine Bouhali, Omar Laouar and Abdelkrim Tahraoui
diazinon, anxiety, depression, pregnancy, thymus atrophy and pancreatic necrosis.

Diazinon is an organophosphate insecticide with broad spectrum of use especially in agriculture. Organophosphorus components inhibit acetylcholinesterase which causes accumulation of acetylcholine in cholinergic synapses that leading to cholinergic stress. Pregnancy is a period known by remakable increase of steroids neuroactive, molecules with therapeutic opportunities especially in neuroprotection. The aim of this study was to examine the response of pregnancy against the neurobehavior and histological effects induced following the subchronic administration of diazinon. Dose of 10mg/kg b.w was injected daily by intraperitoneal way to pregnant rats between the 7th to 14th day of pregnancy. Virgin female rats were also used as basis of comparaison and obey to the same experimental protocol. Thus, recording of sequences of behavior of elevated plus maze was made in 7th, 14th and 21st day of pregnancy. After delivery which occurs at 21st day and lactation which takes 21 days, we tested the effectivenesse of gabaergic agonist, the clonazepam during the forced swimming test, then the prancreas and thymus sampled after decapitation for the histological study. Ours results showed that application of diazinon neurotoxic product during 07 consecutive days caused increase of anxiety behavior that appears to be irreversible in virgin female rats. For cons, the same treatment associated with pregnancy revealed a healing effect after delivery. Inefficiency of clonazepam treatment was observed in virgin female rats treated with diazinon. However this gabaergic agonist was effective in rats which treated with diazinon during pregnancy. Histological exam showed atrophy of thymus and pancreatic necrosis in virgin femele rats, for cons no histological change observed in rats which treated with diazinon during pregnancy. We conclude that pregnancy can play protective role against the alterations induced by diazinon.