Neonatal septicemia is one of the most important cause of morbidity and mortality of neonates. Gram negative bacilli are one of the most important cause of neonatal septicemia being Klebsiella species the most common. Reports of multi resistant bacteria causing neonatal sepsis in developing countries are increasing. So this study will show predominant organisms causing septicemia and their antibiotic susceptibility testing. It was a retrospective study conducted in tertiary care hospital of India for period of 6 months. Samples sent by clinicians to the laboratory were included in the study. Blood culture samples were processed as per standard guidelines and organisms were identified using different biotyping methods. Antibiotic susceptibility was studied by Kirby-bauer disc diffusion method according to CLSI guidelines 2016. 60 isolates were obtained from 131 neonatal blood culture samples. Amongst them, half of isolates were Klebsiella pneumoniae accounting for the most common organism isolated. On antibiotic susceptibility testing, most of gram negative bacilli isolates showed multi drug resistance with complete sensitivity only to Polymyxins. The study emphasize upsurging resistance and importance of judicial use of Polymyxins and Carbapenems.
