Tumor necrosis factor-alpha (TNF- α) is a pleiotropic inflammatory cytokine. The cytokine possesses both growth stimulating properties and growth inhibitory processes, and it appears to have self regulatory properties as well. The cytokine is produced by several types of cells, but especially by macrophage. low levels of TNF-A promote the remodeling or replacement of injured and senescent tissue by stimulating fibroblast growth. The primary role of TNF is in the regulation of immune cells. The study included 100 samples from urban area and 100 sample of Tribal area which is not infected with any other disease. The aim of the study to evaluate the function of TNF- α alleles in population of vindhya region. The diagnosis based on DNA extraction from blood by salting out method. Polymorphism was examined by PCR and RFLP.
