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The identification of the arbuscular mycorrhizal fungi and the evaluation of the mycorrhization level in the roots were performed basing on the isolated spores from the soil and roots samples taken from the rhizosphere of four sugar cane parcels in the region of Dar Gueddari (North western of Morocco). The analysis of the results has shown the presence of different characteristic structures of arbuscular endomycorrhizal in the roots of the sugar cane developed in the prospected sites. The mycorrhization frequency of the sugar cane roots varied from 60% to 100%. The highest intensities of mycorrhization were in the order of 70% in the level of site 2 and 61.53% in the level of site 1 and the lowest intensity was noted in the site 4 (13.3%). Otherwise, the observed arbuscular contents in the level of four sites were respectively 55.32; 66.12; 40.37; 54.03%. The vesicular contents varied from 1.43% (site 4) and 27.91% (site 2). The spore’s density in the rhizosphere of the sugar cane varied between 53 spores/100g (site 3) and 88 (site 1). The identification of the isolated spores allowed to note the presence of 49 species belonging to 8 genera (Glomus, Gigaspora, Acaulospora, Entrophospora, Pacispora, Scutellospora, Ambispora, Rhizophagus), 7 famillies (Glomaceae, Gigasporaceae, and Acaulosporaceae, Pacisporaceae, Scutellosporaceae, Entrophosporaceae, Ambisporaceae) and 4 orders (Glomerales, Gigasporales, Diversisporales, Archaeosporales).