Dna Barcoding And Phylogenetic Inference Of The Endemic Species Onychogomphus Malabarensis (Anisoptera: Gomphidae) Using Mitochondrial Cytochrome Oxidase Subunit I Marker Gene

Research Article
Jisha Krishnan E. K and Sebastian C. D
DOI: 
http://dx.doi.org/10.24327/ijrsr.2018.0901.1576
Subject: 
science
KeyWords: 
Taxonomy, Molecular barcoding, Cytochrome oxidase I gene, Gomphidae, Onychogomphus malabarensis
Abstract: 

The Gomphidae represents one of the fast flying dragonfly families of the insect order Odonata, widely distributed in Palaertic, Ethopian and Oriental regions. Members can be easily diagnosed by having a club like swelling at the base of abdomen (Clubtails) and also black stripes could be seen on their yellow or green coloured thorax. They are habitat specialist and ecologically important as indicators of clean aquatic ecosystem and predators certain dragonflies also. Onychogomphus malabarensis is an endemic Gomphidae member reported only from the forest ecosystem of Palakkad District of Kerala. Here we amplified the cytochrome oxidase I gene and its sequence was deposited in the Gen Bank (KX503058) for future references. The nucleotide BLAST analysis showed that this species is having 100% sequence similarity to Ophiogomphus anomalus reported from America (KX890962). The entire study states that the molecular barcode is a new report to NCBI and their phylogeny inferred their specific taxonomic position among other Gomphidae members.