The bio-fertilizers play a major role in organic farming. In the present study, the various biochemical parameters such as chlorophyll, protein and carbohydrate were estimated at different stages of growth of the two test plants by the application of biofertilizers such as Azospirillum, Phosphobacteria and VAM fungi. The chlorophyll a total chlorophyll content was found to be higher in tomato plants treated with Azospirillum on 30th day and 45th day, but on the 60th day, the chlorophyll a content was found to be higher in plants treated with VAM fungi. In the case of chlorophyll b, it was found that on the 30th day, the content was higher in VAM treated plants and on the 45th day and 60th day, it was observed to be more in Azospirillum treated and combination of fertilizers respectively. In Amaranthusviridis L., the chlorophyll a was found to be higher in plants treated with Azospirillum on the 30th day. Chlorophyll b was found to be higher in plants treated with VAM fungi and total chlorophyll in plants treated with combination of fertilizers. On the 45th day, the chlorophyll a was found to be higher in T4and chlorophyll b and total chlorophyll was higher in plants treated with Phosphobacteria. The protein content in tomato plant was higher in T2 on the 30th day, T4 on the 45th day and T3 on the 60th day. The carbohydrate content was found to be more in T2 on the 30th day, T1 on the 45th day and T4 on the 60th day. In Amaranthusviridis L., the protein content was observed to be higher in T2 on the 30th day and T4 on the 45th day. Similarly, the carbohydrate content was also found to be more in T2 on the 30th day and T3 on the 45th day.
