Effect Of Different Concentrations Of 2,4-D In Nutrient Medium On Growth And Total Phenolic, Flavonoid And Tannin Content Of Callus Obtained From O. Sanctum Leaf Explants

Effect of increasing concentrations of 2,4-D was evaluated on growth and total phenolic, flavonoid and tannin content of callus obtained from leaf explants of Ocimum sanctum. Fresh and dry weight were highest for callus cultured on nutrient medium supplemented with 0.2 µg ml−1 2,4-D. Contents of total flavonoid and tannin increased with increasing concentrations of 2,4-D whereas total phenolic content was highest in callus cultured on nutrient medium enriched with 0.4 µg ml−1 2,4- D. The results of the present study revealed that 2,4-D is required for callus formation from O. sanctum leaf explants. Higher concentrations of it inhibit callus growth but significantly increase the production of flavonoids and tannins.