Background and objective: It has been shown in literature that Fenugreek seeds contain rich amount of steroids and precursor of steroid hormones. The objective of this study was to evaluate the effect of different doses of Fenugreek seeds on mature and perimature rat's vagina. Methodology: A total of 80, healthy virgin Norway albino rats were used. They were divided according to their age into two groups, namely, mature and perimature. Each group was subdivided into subgroups according to the dose of Fenugreek seeds (0.8, 1.6 and 3.2 mg/g bod weight). Results: It has been observed that in experimental perimature subgroups, there was precocious vaginal opening. The appearance of the first estrous phase occurs earlier than the control subgroup, with normal first vaginal cycle and prolong of the second estrous phase. Comparing the cytological results obtained from experimental mature subgroups with their controls, it was obvious that there was ashortening of the vaginal cycle, prolongation in estrous and proestrous phases. In addition, a decrease in the amount of mucus and leukocytes with an increase in the number of cornified and nucleated epithelial cells were identified. Conclusion: The data obtained from this study revealed that crude Fenugreek seeds possessoestrogen and, or progesterone like effect. The vaginal cytology of both mature and Perimature rats treated with Fenugreek seeds was nearly identical, irrespective of the doses used in this study
