Anthropogenic activities like mining, ultimate disposal of treated and untreated waste effluents containing toxic metals as well as metal chelates from different industries, e.g. tannery, steel plants, battery industries, thermal power plants etc. and also the indiscriminate use of heavy metal containing fertilizers and pesticides in agriculture resulted in deterioration of water quality rendering serious environmental problems posing threat on human beings and sustaining aquatic biodiversity. This study deals the effect of different dose concentration (0.5 ppm, 1.0ppm, 1.5ppm and 2.0ppm) of Lead (Pb) and Iron (Fe) on chlorophyll and carotenoid content (mg/kg FW) of Eichhornia crassipes in three consecutive years 2015, 2016 and 2017 in 7days, 14days, 21days and 28days. It was observed that when the dose concentration of Lead or Iron and observation days were increases, the Chl ‘a’ , Chl ‘b’ and carotenoid contents in Eichhornia crassipes were decreases with comparison to control and observation day’s was also higher in 7days in comparison to 14days, 21days and 28days respectively. High concentration of Pb inhibits chlorophyll synthesis by impaired uptake of other essential ions by plants like Mg and Fe or due to increased chlorophyllase activity
