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The composition of sugar factory effluent and its effect on percent germination, growth and seed mycoflora of Arachishypogea were investigated. The effluent was highly acidic.The results showed that lower concentration (10 %) was in favour of germination while there was gradual decrease in germination and seedling growth on higher concentration (50 %). The maximum inhibition in seed germination and seedling growth was found in pure effluent. Percent germination and seed mycoflora were studied on moistened filter paper with different concentration of effluent (10, 50 and 100 %) in a Petri-dish in three replicates and was recorded after seven days. Five seedlings each were randomly selected from the control and the treated plants on the 45th day after planting (DAP) from the earthen-ware pot (6”and 9”). Plant part (shoot and root) was measured and biomass was taken after plants were dried in an oven at 800 C. A total of ten fungal species were recorded from the seeds of Arachis hypogea when treated with different concentration of sugar factory effluent. In 10 % effluent Aspergillu sniger, A. flavus, and mycelia sterilea were recorded. At 50 % seven fungal forms and in100 %, eight fungal species were observed. Percent frequency of mycoflora was increased with the increase of effluent concentration.