A total of 36 crossbred cows with confirmed metritis were divided into 3 groups each comprising 12 cows and treated intrauterine separately with single dose of 100 μg E. coli LPS in 20 ml PBS solution, 500 mg of oyster glycogen in 20 ml PBS solution and 60 ml suspension of 125mg Ciprofloxacin hydrochloride and 150mg Tinidazole / 5ml respectively on first day of oestrus. Metritis was diagnosed on the basis of mucopurulent discharge, thick uterine wall, alkaline pH and white side test. Six cows under each group were inseminated on 2nd day of treatment and the remaining 6 on 2nd day of subsequent oestrus after testing for metritis just before insemination. The result indicated that frequency of occurrence of mucopurulent discharge, thick uterine wall, alkaline pH and positive white side test was 100 % before treatment and 0.00 % after treatment in E. coli LPS treated cows. Corresponding frequencies for oyster glycogen and Ciprofloxacin hydrochloride with Tinidazole treated cows were 100, 100, 100 and 100 % before treatment and 16.67, 16.67, 33.33 and 0.00 % after treatment. The conception rates in the 3 treated groups were 16.66, 0.00 and 0.00 % when inseminations were carried out in the same oestrus and 83.33, 50.00 and 50.00 % in the subsequent oestrus. The study concludes that E. coli LPS has superior immunomodulatory effect over oyster glycogen for treatment of metritis in cows on intrauterine use.
