One of the most comprehensive study that needs an attention to supplement fish diversity data is different kind of adaptations the fishes have adapted due to change in environmental conditions like drought and dry weather which ultimately affects the distribution pattern and use of varied habitats. Present study deals with physiological and behavioral implications of three commonly available air breathing fishes found in selected sites of Digboi region, Assam. The study was conducted in Digboi region for 6 months at different rivers and ponds of the study area from November, 2016 to March, 2017. To check the behavioral and emersion tolerance of amphibious fishes, three commonly available air breathing fishes were collected and passed through an experimental series. Gill ventilation rate was also observed during the period and behavioural changes were noted down during different phases. Among the three species of amphibious air-breathers, all showed significant differences in the pattern of refugia use across the different phases of treatment. However, Clarias batrachus showed better emersion tolerance and can adjust their behavior and physiology in response to emersion, more importantly reverse to a normal state after re-emersion much faster than that of Anabas testidineus and Channa punctatus
