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In the present investigation, the use of low-cost, abundantly available, highly efficient and eco[1]friendly biosorbent Enteromorpha intestinalis ( L.) Knee has been reported as an alternative to the current expensive methods of removing of Methylene Blue (MB) dye from aqueous solution. The effects of different variables such as pH, agitation time, adsorbate concentration, adsorbent dose and agitation speed etc. were investigated and optimal experimental conditions were analysed. The Langmuir isotherm model has given a better conformity than the Freundlich model with 197.74mg/g as maximum adsorption capacity at room temperature. The adsorption of MB on dried biomass of E. intestinalis was confirmed by FTIR, SEM and EDX study, as it showed the change in characterization before and after adsorption. The pseudo- second order model provided a better approximation to the system’s kinetics, while intra particle diffusion study was used to furnish the mechanistic study. Present investigation and comparison with other reported adsorbents concluded that, E. intestinalis may be applied as a low-cost attractive option for removal of MB from aqueous solution