Plant hairs are epidermal appendages of diverse form, structure, functions like protective, supporting and absorbing. Epidermal hairs occur on all parts of a plant, either they persist throughout the life of an organ or they are ephemeral. They classified into different morphological categories. Structurally, they may be subdivided into unicellular or multicellular. They distinguished on the basis of their emergence on the epidermal and sub-epidermal tissues. They have been used in the classification of genera, species in certain families and interspecific hybrids. They may show wide variations within families, within a plant groups and even in the same plant. They act as biomarker and taxonomic significance and also useful to identify the plants even in the powder form. They play a pivotal role in the herbal preparation and enrich the literature on the systematic anatomy of plant. This study reveals the microscopic characteristics of trichomes of Dipteracanthus prostratus (Poir.) Nees.