The present study of Essential Oil of Blumea Oxyodonta DC was designed to evaluate essential oil extraction, phytochemical composition and Anti Microbial Study of essential oil from Blumea Oxyodonta DC collected from Bhramhanwadi, Murud, in Konkan Region. The Essential Oil was extracted by Hydro distillation using Clevenger’s type Apparatus and characterization of essential oil was done by IR, GC-MS and Anti Microbial Activity. Total 81 compounds were identified using Gas Chromatography with Mass Spectroscopy. The main components of essential oil areE,E,Z1,3,12-Nonadecatriene-5,14-diol (9.11%), 1-(+)-Ascorbic Acid 2,6-dihexadecanoate (8.76%), Cyclopropanecarboxylic acid, 2-methyl-, 2,6-di-t-butyl-4-methylphenyl ester (5.89%), Phytol (5.26%), Propanoic acid, 2-methyl-, 2-[3-[(acetyloxy)methyl]oxiranyl]-5-methylphenyl ester (4.02%), 2-Pentadecanone, 6,10,14-trimethyl (3.96%), Propanoic acid, 2-methyl-, 2-[3- [(acetyloxy)methyl]oxiranyl]-5-methylphenyl ester (3.73%), 1-Methyl-1-(m-methylphenyl)-1- silacyclobutane (3.08%), etc.IR confirms the identification of components. Chemical Investigation was done to determine its molecular formula and structure
