In the present investigation, medicinal plants viz Euphorbia hirta and Lucas aspera were selected for their biochemical estimation of primary metabolites (carbohydrates, lipid, protein and phenol) and antioxidant activity was performed using FRAP, Peroxidase assay, LPO. The highest amount of proteins (280mg/gdw) was observed in stem and the minimum content was total soluble sugars (2.2mg/gdw) in root of L.aspera while in E. hirta maximum quantity of lipids (333.3mg/gdw) was observed in stem which was at par with roots and the minimum amount of phenol (0.75mg/gdw) was observed in roots.. Leaves of L. aspera was found to be better antioxidant (150 μmol Fe (II)/g) and leaves in E. hirta (160 μmol Fe (II)/g) when observed by FRAP assay while roots of L. aspera (20.77 mg/g fresh weight) and roots again in E. hirta (16.27 mg/g fresh weight) was more potent when analyzed by LPO. In peroxidase roots of L.aspera (0.56 mg/g fresh weight) and stem in E. hirta (0.176 mg/g fresh weight) were found to be potent.
