The polluted area would be considered as stressed environment which induces the microorganisms to produce several secondary metabolites for their survival between their microbial communities. Prominently fourteen bacterial isolates were isolated from the terrestrial soil samples of steel mills near Coimbatore, among that 7 were pigmented bacteria. All the seven bacterial strains were determined for antagonist activity and BC2-11 strain was a potential strain. The strain was identified as Exiguobacterium profundum BC2-11 through morphological, biochemical, physiological and molecular characterization. The organism produced orange colored metabolite and the crude colored metabolite was extracted using methanol. The crude pigment extract was subjected to HPTLC and it exhibited six peaks, the 5th peak was observed to be highest area of 5973.3 (47.39%) which was the bioactive metabolite. The crude colored metabolite was assessed for antimicrobial profile against clinical pathogens and it revealed highest activity against maximum pathogens. The radical scavenging activity for the colored metabolite was determined through DPPH, metal chelation, reductive power and total antioxidant capacity. The colored metabolite disclosed 50% of inhibitory activity at lower concentrations. Further the colored metabolite will be taken for bioprospecting studies and pharmaceutical applications.
