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Professional stress is one of the posing threat to mental and physical health in modern life. The present study was aimed to evaluate the professional life stress among software professionals in south India. Professional life stress score (PLSS) was assessed based on age, gender, work experience and type of work. Further, resting blood pressure was also assessed. The present findings showed that 85% of the workers had a mild type of stress and 15%of the workers had Moderate Stress. High degree of stress was reported by none of the participants. Unmarried professionals showed a significant (P<0.001) increase in the PLSS score. Based on the type of the designation desk job and human resource recruitment professionals showed a highly significant increase (P<0.001) in the professional life stress score. Moderate Stress groups showed a non significant increase in systolic and diastolic blood pressure compared to the Mild stress group. Based on the present results we suggest that there is a prevalenceof Low to Moderate level of professional life stress among software employees. The management of the software organization must focus on implementing intervention programmes to manage stress