Genetic Diversity of Pseudomonas Isolates From Grassland And Forest Soils of Northern Himalayas Based on 16s Rrna And Genus Specific Region

Research Article
*Asmita Rajwar and Manvika Sahgal
Pseudomonas, ARDRA, 16S rDNA region, Pseudomonas specific gene region, Phylogenetic relationship

The genus Pseudomonas (sensu stricto) represents a group of microorganisms directly involved in functions conferring plant health. Specific detection of Pseudomonas species in the environment may help to gain a more complete understanding of the ecological significance of these microorganisms. In this present study, 16S rDNA and Pseudomonas specific gene region was amplified and it was found out that Pseudomonas specific PCR primer set; which amplify the 990bp region; is more specific for Pseudomonas sensu stricto. In UPGMA dendrogram based on ARDRA of 16S rDNA region, twelve isolates clustered separately from standard strains representing Pseudomonas fluorescens biovar 2, 3, 4 and Pseudomonas fluorescens CHA0 whereas remaining five were intertwined with reference strains used. Out of 17 isolates (PS1, PS2, PS3, PS4, PS5, PS6, PS7, PS8, PS9, PS10, PS11, PS12, PS13, PS14, PS15, PS16 and PS17) Pseudomonas specific region was amplified in 11 isolates (PS1, PS2, PS3, PS4, PS7, PS8, PS9, PS10, PS14, PS15 and PS17). In UPGMA dendrogram based on polymorphism in Pseudomonas specific primer, eleven isolates were placed in two major clusters. Isolates in cluster I could represent different biovars of P. fluorescens or subspecies of P. aeruginosa whereas cluster II represent a novel species in the genus Pseudomonas.