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Ten clinically affected mongrel dogs of either sex and different age groups, weighing 10-20 kg were randomly divided into two groups comprising of five dogs in each group. The dogs of group I were injected with dexmedetomidine @ 20 µg/kg body weight intramuscularly, dogs of group II were injected with dexmedetomidine @ 40 µg/kg body weight intramuscularly. Haemoglobin reduced non-significantly (p>0.05) and TEC reduced significantly (p0.05) in the dogs of both the groups. Significant (p<0.05) increase in the level of Serum Cortisol was observed in both the groups. All the changes were transient and optimum duration was recorded in group I. Based on the findings of the present study, dexmedetomidine @ 20 µg/kg body weight intramuscularly could be suggested for clinical use in dogs.