highlighting of alternaria tenuis conidia in the roots of eucalyptus gomphocephala through the technique of clearing and staining of phillips and haymann (1970)

Research Article
Anasse NOUNSI., Tarik OUCHBANI., Ali OUTCOUMIT., Fatim Ezzahra JANATI IDRISSI., Amina OUAZZANI TOUHAMI., Rachid BENKIRANE and Allal DOUIRA
DOI: 
xxx-xxxx-xxx
Subject: 
science
KeyWords: 
Alternaria tenuis, Eucalyptus, stained roots, endophyte.
Abstract: 

In this study, the Alternaria tenuis conidia was observed in the roots of Eucalyptus plants stained thanks to the technique of Philips and Haymann (1970), used to study the root colonization by endomycorrhizae. The stained roots are bearers of compartmentalized and melanized hyphae, internal and external, and multicellular conidia of Alternaria emanating from the root bark. Different stages of formation of these conidia were observed. Inoculation of Eucalyptus plants with the roots fragments holding Alternaria tenuis could induce root infection that became sporogenous, one month after inoculation, no disease symptoms were observed neither on the plant's roots or on the leaf systems.