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The aim of the present study is to evaluate the anticancer activity of ethyl acetate extract of Terminalia arjuna root. The present investigation was carried out to determine the possible bioactive components of Terminalia arjuna root using GC-MS analysis. Ten bioactive compounds were identified from Terminalia arjuna root. The identified compounds are majorly phenolic derivatives included flavone,6-methoxy flavone, Pentadecanoic acid, methyl ester, Oleic acid, 4- methoxy-2-(1-Phenyl ethyl) phenol, 10-Octadecenic acid, elaidic acid, 3,5,7,2’,4’- pentahydroxyflavone (Morin), Hydrocarbons, Alcoholic compounds, Flavanoids derivatives, Ketones and Fatty acids. The present study is therefore an effort to give detailed information on phytochemical studies and GC-MS analysis of T. arjuna root.