This study was carried out on 36 nurses working in an intensive care (ICU), an emergency care, male and female an internal medicine and surgery departments of general Port Said hospital. The aim of this study was to evaluate impact of an educational program for nurses’ knowledge and practice about Glasgow coma scale (GCS). The data was collected using two tools; “Structured questionnaire sheet” which is consisted of 15 questions related to socio-demographic characteristics, and knowledge about GCS , “observation checklist” were related to practice about GCS . The results of this study indicated that there are statistically significant improvements immediately after program implementation regarding nurses’ knowledge and practice about GCS. Moreover, it was also found that statistically significant relation between nurses’ knowledge and practice, and there is no statistically significant association between the changes in the scores of either knowledge or practice and socio-demographic characteristics. Finally the program had succeeded in inducing statistically significant improvements of nurses’ knowledge and practice about GCS. Therefore, it can be concluded from the results of the present study that this training program for nurses had a positive impact on their knowledge and practice.