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The purpose of the present research was to compare a serum zinc concentration before and after acute exercise. The sample of the research consisted of 14 physical education students of Diyala University with the experience of participating in national long-distance track events who voluntarily participated in the study (23.00 ± 1.78 years old; 174.36 ± 6.24 cm height; 66.58 ± 5.76 kg weight). The exercise loads were performed with 1 days interval. Before, and after test Serum zinc of the participants were measured. In addition, blood pressure, of the participants were calculated before and after exercise. The data was analyzed in SPSS 13. The mean and standard deviation of all the variables were calculated. T test was applied to examine significant differences between the variables. A significant difference was observed in serum zinc of the participants before and after exercise. Also, there was elevated in high pressure after exercise. Negative significant correlation (P<0.05) was found between the concentration of zinc and high blood after exercise