L-Asparaginase has received increased awareness in current years for its anti carcinogenic potential. In the present work a total of 5 soil samples were collected from various places near to Aurangabad, from these soil samples 12 isolates were identified as Bacillus licheniformis (5), Bacillus alvei (2), Bacillus megaterium (1), Bacillus cereus (1), Bacillus circulans (1) and Bacillus subtilis (2). The optimization study for the process parameters was given useful information about the production of the enzyme maximally. Potential L-asparginase producer isolates were showed the maximum activity at pH 7 and temperature at 37°C. After that the isolates were exposed to the UV treatment for 5, 10 and 15 min for strain improvement and among them Asp11 (Bacillus subtilis) was found useful strain for the commercial production of the L-Asparaginase. This highly potential species can be used for the commercial production of the enzyme which is having immense medical applications as it is one of the promising treatments for the threatening disease called as cancer.
