The present study was conducted to investigate effect of type and concentration of different sugars as carbohydrates sources on callus induction, direct and indirect organogenesis using roots explants of Albizialebbeck Benth. Sterile seeds of A. lebbeck were germinated on MS free medium. Roots explants were taken from 10-12 days old seedlings and transferred to MS medium contained 4.0 g.l-1 charcoal with sugars: glucose, maltose and sucrose individually at :0.0, 20, 30 and 40 (g.l-1 ). Data were recorded according to the callus induction percentage, numbers and lengths of the regenerated roots and shoots. Results showed highest percentage of callus induction (100%) was obtained at 30g.l-1 glucose, 20 and 30 g.l-1 maltose ; 20, 30 and 40 g.l-1 of sucrose, whereas glucose at the concentration 20g.l-1 gave highest mean of root number (9.64 roots), shoot number (7.0 shoots) and number of leaves (5.71 leaves/shoot), while the highest means of root length (11.9 and 11.7 mm) were recorded in sucrose at 20 and 40 g.l-1 respectively, and longest shoot length (19.9 mm) was recorded in 30 g.l-1 of glucose. Induced callus was sub-cultured on MS medium without charcoal and contained TDZ (1.0, 2.0 and 3.0 mg.l-1 ) for regeneration. Results showed the highest fresh and dry weights (4540 279 mg respectively), and highest number of regenerated shoots (17.8 shoots) were obtained at 3.0 g.l-1 of TDZ.
