In these decades, drastic attention in the natural products especially mentioned Ayurvedas and other conventional systems of medicines has been observed. Drugs obtained from plant have been measured much safer and possess a tremendous utility in the treatment of various diseases. Medicinal plants are called molecular factory, as they have ability to produce vastrange of compounds derived from them thus termed as "Bioactive compounds". The plants bears different phytochemicals known to early civilizations for various curative properties. Researches in these area sare assisting in depth knowledge about the mechanisms, their role and formation of secondary metabolites and nutraceuticals of importance. Buteamonosperma is significant medicinal plant having several medicinal values like antimicrobial, antioxidant and anticancer agents. It is an important arid zone flora normally consumed as herbal drugs for treatment in pain and inflammation, including othermetabolic disorders such as diabetes and obesity.During present investigations,phytosterols was characterized and quantified in vivofrom B. monosperma. Chromatographic and spectral studies were used to confirm Phytosterols in which ß-sitosterol, campesterol and stigmasterol were confirmedthrough TLC, IR and GC-MS. GC-MS profiling showed presence of 49 various compounds.It was observed that maxium area was occupied by Hexadenoic acid (54.4%).
