Amylases are one of the main enzymes used in industry. Such enzymes hydrolyze the starch molecules into polymers composed of glucose units. Amylases have potential application in wide number of industrial processes such as food, fermentation and pharmaceutical industries. Microbes are the most preferred sources of enzymes due to their broad biochemical diversity. The present study focused with the isolation of amylase producers from soil receiving kitchen and agricultural waste. From the 9 soil samples we had isolated 65 isolates out of which 29 isolates were selected based on amylase activity as clear zone on starch agar. Out of 29 isolates 10 isolates show excellent zone of hydrolysis in the secondary screening. The isolates showed prominent activity were further identified by standard conventional methods, which showed most of them belonging to genus Bacillus followed by Pseudomonas spp., Serratia marcescens and Staphylococcus aureus. This study can be further used for the study of amylase on different parameters.
