Tinospora cordifolia is an important medicinal plant belonging to family Menispermaceae. Flavonoids were isolated from leaves, stem, seeds and in vitro callus of the species. The dried samples were separately soxhlet extracted in 80% methanol and then re-extracted with petroleum ether, diethyl ether and ethyl acetate. The fraction was concentrated and subjected to thin layer chromatography. The Rf value of isolated compounds and standard was calculated, which confirm the presence of quercetin, luteolin and kaempferol. The purified material was subjected to its IR spectra, High-performance liquid chromatography peak of isolated quercetin at retention time 2.652 min was observed. This study is also of practical importance because flavonoids are an important ingredient of T. cordifolia.
