Selected plants for study were Manilkarazapota L. and Madhucalongifolia L. Both plant belongs to family Sapotaceae and have many medicinal values. Phytosterols from Manilkarazapota L. and Madhucalongifolia L. were identified and quantified in vivo. Phytosterols were identified using chromatographic and spectral studies. ß-sitosterol, campesterol and stigmasterol were identified by IR and GC-MS. GC-MS profiling showed various compounds. In Manilkarazapota L. 1,3- Butanedione, 1-Phenyl found in highest amount (area of % 31.49%), while 5H,10H-Dipyrrolo[1,2- A:1',2'-D]Pyrazine-5,10-Dione, Octahydro-, (5AS-CIS) and Pyrimidine, 2-phenyl-4-hydroxy-5- dimethylamino found in minimum amount (area of % 0.16 %). In Madhucalongifolia L. Ethyl Oleate found in highest amount (area of % 22.72%), while 2-Ethylhexyl salicylate found in lowest amount (area of % 0.16). It is the first report on phytosterols from the experimental plants.
