isolation and screening marine bacterial for the production of amylase and phosphate solubilization

Research Article
Lekshmi S. Baby., Mary Franceena., Lekshmi M and Ayona Jayadev*
DOI: 
xxx-xxxx-xxx
Subject: 
science
KeyWords: 
Amylase production, Phosphate solubilization, Marine bacteria, Optimization
Abstract: 

Marine environment, which is unique in many ways harbors microorganisms with distinctive potentials. Researchers are now concentrating on products produced by microbes of special environment for various reasons. This work was carried out to screen and isolate marine bacteria with amylase production and phosphate solubilization capacities. A total of 12 bacterial strains were isolated from three sampling sites along the coast of Arabian Sea. Six of the isolated bacterial strains showed positive amylase activity. A considerable production was there at pH 4. The highest amylase producer is Bb1 and the organism produced the least is Bb3. Tb3 and Bb2, of whose production was low in acidic as well as neutral pH, increased manifold in alkaline pH. All bacterial strains invariably showed the greatest activity at alkaline pH, 9. In the case of the temperature of incubation, except Bb1 and Bb2, other strains showed maximum activity at 37°C. Of the six strains, Ab2 showed maximum activity (2.45 Units/mL) at 37°C. This was followed by Bb3 (2.15 Units/mL) and Ab1 (1.98 Units/mL). Strain Ab2, the maximum amylase producer, produced the least amount at 20°C. Except the isolate Bb1, all isolates showed the highest production of amylase at the third day of incubation. Of all the strains, Ab2 produces the maximum amount of enzyme (2.45 Units/mL). Out of 12 strains, seven bacterial strains showed clear zone around their colonies when cultured in media substituted with ticalcium phosphate, thus showing phosphate solubilization ability. Maximum clearance zone was given by isolates from near effluent discharge site (Tb3 and Tb4 with a zone size of 8 and 8.1cm respectively). Isolates Bb2 and Bb4 did not produce clearance zone and the other isolates from the same sampling site (near a harbor).