In the present study, 20 different bacterial species were isolated from offshore seawater and sediment of Koodankulam coast, Gulf of Mannar, South East coast of India. The isolated bacteria were subjected to screening by cross streaking method against ten different human pathogenic bacteria like Staphylococcus aureus, Micrococcus luteus, Streptococcus pyogenes, Bacillus subtilis, Enterobacter faecalis, Escherichia coli, Klebsiella pneumonia, Shigella sonnei, Salmonella typhimurium, Vibrio cholerae and the isolated bacteria. The results showed potential activity against three different human bacterial pathogen namely Micrococcus luteus, Staphylococcus aureus and Klebsiella pneumonia. The culture was extracted using ethyl acetate, n-butanol, hexane, chloroform and screened for antibacterial activity by disc diffusion method. Among the different solvents ethyl extract showed the broad spectrum of activity against all ten human pathogens (ST5). The strain ST5 was subjected for morphological and potential molecular identification based on 16s rDNA sequencing.
